Hybrid Polyketides from a Hydractinia-Associated Cladosporium sphaerospermum SW67 and Their Putative Biosynthetic Origin
- Authors
- Lee, Seoung Rak; Lee, Dahae; Eom, Hee Jeong; Rischer, Maja; Ko, Yoon-Joo; Kang, Ki Sung; Kim, Chung Sub; Beemelmanns, Christine; Kim, Ki Hyun
- Issue Date
- Nov-2019
- Publisher
- MDPI
- Keywords
- hybrid polyketides; tetramic acid; Cladosporium sphaerospermum; hybrid PKS-NRPS; LLC-PK1 cells
- Citation
- MARINE DRUGS, v.17, no.11
- Journal Title
- MARINE DRUGS
- Volume
- 17
- Number
- 11
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/18011
- DOI
- 10.3390/md17110606
- ISSN
- 1660-3397
- Abstract
- Five hybrid polyketides (1a, 1b, and 2-4) containing tetramic acid core including a new hybrid polyketide, cladosin L (1), were isolated from the marine fungus Cladosporium sphaerospermum SW67, which was isolated from the marine hydroid polyp of Hydractinia echinata. The hybrid polyketides were isolated as a pair of interconverting geometric isomers. The structure of 1 was determined based on 1D and 2D NMR spectroscopic and HR-ESIMS analyses. Its absolute configuration was established by quantum chemical electronic circular dichroism (ECD) calculations and modified Mosher's method. Tetramic acid-containing compounds are reported to be derived from a hybrid PKS-NRPS, which was also proved by analyzing our C-13-labeling data. We investigated whether compounds 1-4 could prevent cell damage induced by cisplatin, a platinum-based anticancer drug, in LLC-PK1 cells. Co-treatment with 2 and 3 ameliorated the damage of LLC-PK1 cells induced by 25 mu M of cisplatin. In particular, the effect of compound 2 at 100 mu M (cell viability, 90.68 +/- 0.81%) was similar to the recovered cell viability of 88.23 +/- 0.25% with 500 mu M N-acetylcysteine (NAC), a positive control.
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