Sanguiin H-11 from Sanguisorbae radix protects HT22 murine hippocampal cells against glutamate-induced death
- Authors
- Song, Ji Hoon; Kim, Song-Yi; Hwang, Gwi Seo; Kim, Youn-Sub; Kim, Hyun Young; Kang, Ki Sung
- Issue Date
- 15-Jan-2019
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Sanguiin H-11 (SH-11); Glutamate; Mitogen-activated protein kinase; Oxidative stress; HT22 cells
- Citation
- BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, v.29, no.2, pp.252 - 256
- Journal Title
- BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
- Volume
- 29
- Number
- 2
- Start Page
- 252
- End Page
- 256
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1980
- DOI
- 10.1016/j.bmcl.2018.11.042
- ISSN
- 0960-894X
- Abstract
- Excessive glutamate level induces neuronal death in acute brain injuries and chronic neurodegenerative diseases. Natural compounds from medicinal and food plants have been attracting interest as a treatment for neurological disorders. Sanguiin H-11 (SH-11), a hydrolysable ellagitannin, inhibits neutrophil movement and nitric oxide-production. However, its neuroprotective effect has not been studied. Therefore, the present study examined the protective effect of SH-11 from Sanguisorbae radix and its mechanism against glutamate-induced death in HT22 cells. Our results showed that SH-11 possessed a strong antioxidant activity and prevented glutamate-induced death in HT22 cells. As a strong antioxidant, SH-11 significantly reduced glutamate-induced increases in intracellular reactive oxygen species accumulation and calcium ion influx. Western blotting analysis showed that glutamate-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including extracellular signal-related kinases 1/2, c-Jun N-terminal kinase, and p38, was significantly decreased by SH-11. Furthermore, SH-11 significantly decreased the number of annexin V-positive HT22 cells, which is indicating apoptotic cell death. In conclusion, our results suggested that SH-11 exerted a potent neuroprotective activity against glutamate-mediated apoptotic cell death by inhibiting oxidative stress-mediated MAPK activation.
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