한수대보원이 포유동물인 생쥐 모델에서 골 손실 및 RANKL 유도 파골세포 분화에 미치는 영향Effects of Hansu-Daebowon (HDW) on RANKL-induced Osteoclast Differentiation and Bone Loss in Mammal Model
- Other Titles
- Effects of Hansu-Daebowon (HDW) on RANKL-induced Osteoclast Differentiation and Bone Loss in Mammal Model
- Authors
- 장시성; 류홍선; 전찬용; 황귀서
- Issue Date
- 2019
- Publisher
- 대한한방내과학회
- Keywords
- Hansu-Daebowon (HDW); osteoclast differentiation; TRAP; cathepsin K; NFATc1; bone loss; bone resorption
- Citation
- 대한한방내과학회지, v.40, no.1, pp.58 - 69
- Journal Title
- 대한한방내과학회지
- Volume
- 40
- Number
- 1
- Start Page
- 58
- End Page
- 69
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/2557
- ISSN
- 1226-9174
- Abstract
- Objective: This study investigated the effects of Hansu-Daebowon (HDW) on bone resorption in vitro and bone loss in vivo.
Methods: Osteoclast differentiation was measured by counting TRAP (+) MNC formed from RAW 264.7 in the presence of RANKL. Bone pit formation was determined in an artificial bone slice loaded with RANKL-stimulated osteoclasts. To elucidate the mechanisms of the inhibitory effects of HDW on bone resorption and osteoclast differentiation, osteoclastogenic genes (i.e. TRAP, MMP-9, NFATc1, c-Fos, and Cathepsin K) were measured using real time PCR. Furthermore, bone loss was observed using micro-CT in an LPS-treated mammal model.
Results: HDW inhibited the bone pit formation in vitro and inhibited bone loss in vivo. Moreover, HDW decreased the number of TRAP (+) MNCs in the presence of RANKL, and HDW inhibited the expressions of cathepsin K, MMP-9, TRAP, NFATc1, and c-Fos in the osteoclasts.
Conclusion: HDW exerts inhibitory effects on bone loss and bone resorption resulting from the inhibitions of osteoclast differentiation and osteoclastogenic gene expression.
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