Nitrilotriacetic acid-end-functionalized polycaprolactone as a template for polymer-protein nanocarriers
- Authors
- Jose, Leeja; Hwang, Aran; Lee, Chaeyeon; Shim, KyuHwan; Song, Jae Kwang; An, Seong Soo A.; Paik, Hyun-jong
- Issue Date
- 7-Mar-2020
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- POLYMER CHEMISTRY, v.11, no.9, pp.1580 - 1588
- Journal Title
- POLYMER CHEMISTRY
- Volume
- 11
- Number
- 9
- Start Page
- 1580
- End Page
- 1588
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/26078
- DOI
- 10.1039/c9py01663e
- ISSN
- 1759-9954
- Abstract
- Diverse polymer-protein nanostructures were introduced as promising platforms for biomedical applications based on their unification of biocompatibility, the bespoke functionalities of proteins, and the structural integrity of polymers. Previously, the polymer-templated protein nanoball (PTPNB) system with Ni2+-nitrilotriacetic acid-end-functionalized polystyrene (Ni2+-NTA-PS) and His(6)-GFP was reported. These synthesized nanostructures maintained a high protein activity and protein orientation through a soft and strong specific Ni2+-NTA/histidine synergy between the protein and the polymer. However, the toxicity of polystyrene (PS) was a major limitation of the PTPNB system for biomedical applications. Herein, biocompatible polymer-protein nanocarriers were developed by synthesizing nickel-complexed nitrilotriacetic acid-end-functionalized polycaprolactone (Ni2+-NTA-PCL). The potentiality of the current system was the preparation of polymer-protein hybrid nanocarriers with the loaded doxorubicin (DOX) in a one-pot process. Finally, the capability of DOX-loaded GFP/PCL as a therapeutic vehicle was verified by the cellular internalization and cytotoxicity test with neuroblastoma SH-SY5Y cells.
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