A Convenient Colorimetric Bacteria Detection Method Utilizing Chitosan-Coated Magnetic Nanoparticles
- Authors
- Thao Nguyen Le; Tai Duc Tran; Moon Il Kim
- Issue Date
- Jan-2020
- Publisher
- MDPI
- Keywords
- colorimetric bacteria detection; chitosan-coated magnetic nanoparticles; peroxidase-like activity; Escherichia coli; Staphylococcus aureus
- Citation
- NANOMATERIALS, v.10, no.1
- Journal Title
- NANOMATERIALS
- Volume
- 10
- Number
- 1
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/26188
- DOI
- 10.3390/nano10010092
- ISSN
- 2079-4991
- Abstract
- An effective novel strategy to detect bacteria is promising because it may improve human health by allowing early diagnosis and timely treatment of bacterial infections. Here, we report a simple, reliable, and economical colorimetric assay using the peroxidase-like activity of chitosan-coated iron oxide magnetic nanoparticles (CS-MNPs). When CS-MNPs are incubated with a sample containing bacterial cells such as the gram-negative Escherichia coli or the gram-positive Staphylococcus aureus, the negatively-charged bacterial membrane interacts with positively-charged chitosan on the surface of CS-MNPs, thus resulting in significant reduction of their peroxidase-like activity presumably by a hindrance in the accessibility of the negatively charged substrate, 2-2 '-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) to the positively-charged CS-MNPs. This simple colorimetric strategy allowed the rapid detection of bacterial cells down to 10(4) CFU mL(-1) by the naked eye and 10(2) CFU mL(-1) by spectrophotometry within 10 min. Based on the results, we anticipate that the CS-MNPs-based assay has great potential for the on-site diagnosis of bacterial infections in facility-limited or point-of-care testing (POCT) environments.
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