Safety evaluation of bacteriophages for application as sanitizers박테리오파지의 살균소독제 응용을 위한 안전성 평가
- Other Titles
- 박테리오파지의 살균소독제 응용을 위한 안전성 평가
- Authors
- 박도원; 이영덕; 박종현
- Issue Date
- Feb-2020
- Publisher
- 한국식품과학회
- Keywords
- bacteriophage; endotoxin; LAL assay; cell cytotoxicity
- Citation
- 한국식품과학회지, v.52, no.1, pp.109 - 112
- Journal Title
- 한국식품과학회지
- Volume
- 52
- Number
- 1
- Start Page
- 109
- End Page
- 112
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/26268
- ISSN
- 0367-6293
- Abstract
- To evaluate the safety of bacteriophages for application of sanitizer, endotoxin content and cell cytotoxicity of two Escherichia coli and four Staphylococcus aureus phages were determined. Endotoxin ratio was determined by the Limulus amebocyte lysate (LAL) assay as a test for representative biological endotoxin content. The average endotoxin average content of the 9 log PFU/mL lysate was 18.6 EU/mL and that of the 10 log PFU/mL lysate was 5.9 EU/mL, suggesting that the phage lysate was not suitable for clinical applications, but suitable for food pathogen control applications. To confirm the cell cytotoxicity of the phage lysates, MTT assay was performed using Raw 264.7 cells treated with 9 log PFU/mL phages. Results of the assay indicated that the phage lysates did not significantly decrease the cell viability (p>0.05). These results indicated that bacteriophages would be suitable as a food safety sanitizer.
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