Inhibitory effects of orientin in mast cell-mediated allergic inflammation
- Authors
- Dhakal H.; Lee S.; Choi J.K.; Kwon T.K.; Khang D.; Kim S.-H.
- Issue Date
- Aug-2020
- Publisher
- Springer
- Keywords
- Allergic inflammation; Histamine; Mast cell; Orientin; Passive cutaneous anaphylaxis
- Citation
- Pharmacological Reports, v.72, no.4, pp.1002 - 1010
- Journal Title
- Pharmacological Reports
- Volume
- 72
- Number
- 4
- Start Page
- 1002
- End Page
- 1010
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/26326
- DOI
- 10.1007/s43440-019-00048-3
- ISSN
- 1734-1140
- Abstract
- Background: Mast cells are immune effector cells mediating allergic inflammation by the secretion of inflammatory mediators such as histamine and pro-inflammatory cytokines. Orientin is a naturally occurring bioactive flavonoid that possesses diverse biological properties, including anti-inflammation, anti-oxidative, anti-tumor, and cardio protection. The objective of this study was to rule out the effectiveness of orientin in mast cell-mediated allergic inflammation. Methods: In this study, in vitro effects of orientin were evaluated in RBL-2H3, mouse bone marrow-derived mast cells, rat peritoneal mast cells, and in vivo effects were evaluated by inducing passive cutaneous anaphylaxis (PCA) in Imprinting Control Region (ICR) mice. Results: Findings show that orientin suppressed the immunoglobulin E (IgE)-mediated mast cell degranulation by reducing intracellular calcium level in a concentration-dependent manner. Orientin suppressed the secretion of pro-inflammatory cytokines in mast cells. This inhibitory effects of orientin was through inhibition of FcεRI-mediated signaling proteins. In addition, oral administration of orientin suppressed the IgE-mediated PCA reactions in a dose-dependent manner, which was evidenced by reduced Evan’s blue pigmentation and ear swelling. Conclusions: Based on these findings, we suggest that orientin might have potential to alleviate allergic reaction and mast cell-mediated allergic disease. © 2020, Maj Institute of Pharmacology Polish Academy of Sciences.
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