Unique triterpenoid of jujube root protects cisplatin-induced damage in kidney epithelial LLC-PK1 cells via autophagy regulation
- Authors
- Lee D.; Kang K.B.; Kim H.W.; Park J.S.; Hwang G.S.; Kang K.S.; Choi S.; Yamabe N.; Kim K.H.
- Issue Date
- Mar-2020
- Publisher
- MDPI AG
- Keywords
- Autophagy; Cisplatin; Nephrotoxicity; Ziziphus jujuba
- Citation
- Nutrients, v.12, no.3
- Journal Title
- Nutrients
- Volume
- 12
- Number
- 3
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/26387
- DOI
- 10.3390/nu12030677
- ISSN
- 2072-6643
- Abstract
- Chronic exposure to cisplatin is associated with irreversible kidney impairment. In this present study, we explored the protective effects of 3-dehydroxyceanothetric acid 2-methyl ester (3DC2ME) isolated from roots of jujube (Ziziphus jujuba, Rhamnaceae) against cisplatin-induced damage in vitro. In kidney epithelial LLC-PK1 cells, western blotting and staining with specific autophagy epifluorescent dye CytoID were used to determine the molecular pathways involving autophagy. Treatment with 3DC2ME reduced the increased Cyto-ID-stained autophagic vesicles and reversed the protein expressions of 5’ AMP-activated protein kinase subunit β-1 (AMPK)/mammalian target of rapamycin (mTOR)-dependent signaling pathway in cisplatin-induced cell death. Additionally, treatment with autophagy inhibitor 3-methyladenine (3-MA) and with or without 3DC2ME attenuated the cisplatin-induced apoptosis. Although further research is necessary to substantiate the effects, we evaluated the potential mechanism of action of 3DC2ME as an adjuvant for cancer patients. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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