Label-free sensitive detection of MUC1 using a liquid crystal based-system
- Authors
- Duong, Thai Duong Song; Jang, Chang-Hyun
- Issue Date
- Sep-2020
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- Liquid crystal (LC); 4-cyano-4MODIFIER LETTER PRIME-pentylbiphenyl (5CB); mucin 1 (MUC1); aptamer
- Citation
- LIQUID CRYSTALS, v.47, no.12, pp.1784 - 1793
- Journal Title
- LIQUID CRYSTALS
- Volume
- 47
- Number
- 12
- Start Page
- 1784
- End Page
- 1793
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/26454
- DOI
- 10.1080/02678292.2020.1729426
- ISSN
- 0267-8292
- Abstract
- This study reports the development of a novel label-free aptasensor which uses the orientation properties of nematic liquid crystals (LCs) to detect mucin 1 (MUC1) is developed. The sensor is made of immobilised amine-functionalised aptamers on the surface, with LCs serving as response elements. When MUC1 interacts with the immobilised aptamers, it forms a complex that disrupts the LC orientation, taking them from homeotropic to random alignment. This change in the orientation of LCs is easily observed as a shift in the optical LC image from dark to bright using a polarised light microscope. This sensing system is highly sensitive (detection limit of 5 fg mL(-1)) and exhibits good specificity for detection of MUC1 without labelling or an additional amplification step.
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Collections - 바이오나노대학 > 나노화학과 > 1. Journal Articles
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