Detection of catalase activity with aldehyde-doped liquid crystals confined in microcapillaries
- Authors
- Rim, Jinseob; Jang, Chang-Hyun
- Issue Date
- 1-Nov-2018
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Liquid crystal; 4-Cyano-4 ' -pentylbiphenyl (5 C B); Microcapillary; Hydrogen peroxide; Catalase
- Citation
- ANALYTICAL BIOCHEMISTRY, v.560, pp.19 - 23
- Journal Title
- ANALYTICAL BIOCHEMISTRY
- Volume
- 560
- Start Page
- 19
- End Page
- 23
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3091
- DOI
- 10.1016/j.ab.2018.08.010
- ISSN
- 0003-2697
- Abstract
- In this study, a simple, rapid, and label-free sensor was developed for detecting the enzymatic activity of catalase (CAT) with liquid crystals (LCs) confined in microcapillaries. Inside a microcapillary functionalized with n-octyltrichlorosilane, aldehyde-doped LCs anchored radially so that a pattern of straight lines was observed under a polarized optical microscope (POM). However, once hydrogen peroxide (HP) oxidized the aldehyde into carboxylic acid, which has surface activity, the orientation of the LCs at the interface changed, resulting in a distinct pattern change, from straight to crossed. In this system, the enzymatic activity of CAT could be detected as it inhibits the oxidation by decomposing HP; as a result, the pattern changed back to the straight one. From the orientational and optical shift, the enzymatic activity of CAT was detected up to a concentration of 0.8 fM under mild experimental conditions and 8 aM at pH 9.0. This result suggests the need for further study of microcapillary systems to develop simple and sensitive sensors for biochemical interactions.
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