Tetrahydrocurcumin Enhances Islet Cell Function and Attenuates Apoptosis in Mouse Islets
- Authors
- Kim, S. S.; Jang, H. J.; Oh, M. Y.; Lee, J. H.; Kang, K. S.
- Issue Date
- Nov-2018
- Publisher
- ELSEVIER SCIENCE INC
- Citation
- TRANSPLANTATION PROCEEDINGS, v.50, no.9, pp.2847 - 2853
- Journal Title
- TRANSPLANTATION PROCEEDINGS
- Volume
- 50
- Number
- 9
- Start Page
- 2847
- End Page
- 2853
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3122
- DOI
- 10.1016/j.transproceed.2018.03.033
- ISSN
- 0041-1345
- Abstract
- Background. The transplantation of isolated pancreatic islets is a promising treatment for diabetes. Curcumin has been used for its pharmacologic effects, such as antidiabetic and anti-inflammatory activities. Tetrahydrocurcumin (THC), one of the major metabolites of curcumin, has been reported to have antioxidant and anti-inflammatory activities. This study examines the hypothesis that preoperative THC treatment can attenuate ischemic damage and apoptosis before islet transplantation. Methods. Islets isolated from Balb/c mice were randomly divided into 2 groups and cultured in medium supplemented with or without THC. In vitro islet viability and function were assessed. After treatment with a cytokine cocktail consisting of tumor necrosis factor-alpha, interferon-beta, and interleukin-1 beta, islet cell viability, function, and apoptotic status were determined. Proteins related to apoptosis were analyzed using INS-1 cell after streptozocin treatment. Results. There was no difference in cell viability between the 2 groups. Islets cultured in the medium supplemented with THC showed 1.3-fold higher glucose-induced insulin secretion than the islets cultured in the medium without THC. After treatment with a cytokine cocktail, glucose-induced insulin release, and NO of the islets were significantly improved in THC-treated islets compared with islets not treated with THC. Apoptosis was significantly decreased, and B-cell lymphoma-2 was elevated in the THC-treated group. The streptozocin-treated INS-1 cell produced significantly higher levels of and B-cell lymphoma-2-associated X protein, caspase-3, and caspase-9 than INS-1 treated with THC. Conclusions. These results suggest that preoperative THC administration enhances islet function before transplantation and attenuates the cytokine-induced damage associated with apoptosis.
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