Orientation Controlled Protein Nanocapsules by Enzymatic Removal of a Polymer Template
- Authors
- Lee, Chaeyeon; Hwang, Aran; Jose, Leeja; Park, Ji Hyun; Song, Jae Kwang; Shim, KyuHwan; An, Seong Soo A.; Paik, Hyun-jong
- Issue Date
- Nov-2018
- Publisher
- AMER CHEMICAL SOC
- Citation
- BIOMACROMOLECULES, v.19, no.11, pp.4219 - 4227
- Journal Title
- BIOMACROMOLECULES
- Volume
- 19
- Number
- 11
- Start Page
- 4219
- End Page
- 4227
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3170
- DOI
- 10.1021/acs.biomac.8b00965
- ISSN
- 1525-7797
- Abstract
- Protein nanocapsules are potentially useful as functional nanocarriers because of their hollow structure and high biocompatibility and the intrinsic activity of their protein constituents. However, the development of a facile method for the preparation of oriented nanocapsules that retain their protein activity has been challenging. Here we describe the preparation of protein nanocapsules through the enzymatic. removal of polymer templates. Nickel(II) nitrilotriacetic acid-end-functionalized poly(lactic acid) (Ni2+-NTA-PLA) was introduced as a polymeric template to immobilize hexa-histidine-tagged green fluorescence protein (His6-GFP) with consistent orientation. Following protein cross-linking and core-degradation, various measurements as a function of degradation time indicated the formation of hollow structures. We also demonstrated orientational control and activity preservation of the protein after capsule preparation. Protein nanocapsules prepared by this method can act as functional containers, taking advantage of the intrinsic function of their constituent proteins without additional modification.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 바이오나노대학 > 바이오나노학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3170)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.