Imaging DNA single-strand breaks generated by reactive oxygen species using a liquid crystal-based sensor
- Authors
- Kim, Hyeong Jin; Jang, Chang-Hyun
- Issue Date
- 1-Sep-2018
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Liquid crystal (LC); 4-Cyano-4 ' -pentylbiphenyl (5CB); DNA single-strand breaks (SSBs); Fenton reaction; Reactive oxygen species (ROS); Orientational transition
- Citation
- ANALYTICAL BIOCHEMISTRY, v.556, pp.1 - 6
- Journal Title
- ANALYTICAL BIOCHEMISTRY
- Volume
- 556
- Start Page
- 1
- End Page
- 6
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3351
- DOI
- 10.1016/j.ab.2018.06.009
- ISSN
- 0003-2697
- Abstract
- DNA single-strand breaks (SSBs) have attracted much interest since they are highly related to carcinogenesis and ageing. Herein, we report a new liquid crystal (LC)-based sensor for the detection of DNA SSBs generated by reactive oxygen species (ROS) created from the Fenton reaction. The adsorption of single-stranded DNA (ssDNA) onto the cationic surfactant-laden aqueous/LC interface interferes with the surfactant layer, inducing a planar orientation of the LCs. However, the DNA SSBs generated by the Fenton reaction-produced ROS result in a decrease of the electrostatic interactions between the ssDNA and cationic surfactant molecules, causing rearrangement of the surfactant layer and reorientation of the LCs back to a homeotropic alignment. The changes in orientation of the LCs caused by the DNA SSBs are simply converted and observed as a shift from a bright optical image of the LCs to a dark one under a polarized light microscope. With this simple LC-based approach, the DNA SSBs could be detected more effectively and rapidly without any complex instrumentation or intricate processes. Therefore, our research provides a novel strategy for the detection of DNA damage as well as better insight into the DNA-damaging process.
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