Fluorescence Enhancement Using Bimetal Surface Plasmon-Coupled Emission from 5-Carboxyfluorescein (FAM)
- Authors
- Nhu Hoa Thi Tran; Kieu The Loan Trinh; Lee, Jun-Ho; Yoon, Won Jung; Ju, Heongkyu
- Issue Date
- Sep-2018
- Publisher
- MDPI
- Keywords
- fluorescence enhancement; surface plasmon resonance; 5-carboxyfluorescein; nano-biophotonics; optical biosensors
- Citation
- MICROMACHINES, v.9, no.9
- Journal Title
- MICROMACHINES
- Volume
- 9
- Number
- 9
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3401
- DOI
- 10.3390/mi9090460
- ISSN
- 2072-666X
- Abstract
- We demonstrate the enhancement of fluorescence emission from a dye, 5-carboxyfluorescein (FAM), which couples with surface plasmons at the spectral channels of excitation and emission. Experiments and calculations revealed that bimetallic (gold-silver) plasmon, as compared to the monometallic ones, allowed such coupling to be enhanced, at both the spectral channels. We achieved the maximum fluorescence enhancement level of 46.5-fold, with markedly high reproducibility (coefficient of variation similar to 0.5%) at a FAM concentration of 10 nM. We also found that higher fluorescence enhancement was more likely to be reproducible. This encourages the use of this technology for practical applications in fluorescence-based biochemical assays. Moreover, we investigated a FAM concentration-dependent enhancement of fluorescence. It was found that fluorescence enhancement decreased and saturated at above 10 nM concentration possibly due to partial photo-bleaching of FAM molecules.
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Collections - 바이오나노대학 > 나노물리학과 > 1. Journal Articles
- 공과대학 > 화공생명공학과 > 1. Journal Articles
- 산업·환경대학원 > 산업환경공학과 > 1. Journal Articles
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