Chemical Identification of Isoflavonoids from a Termite-Associated Streptomyces sp RB1 and Their Neuroprotective Effects in Murine Hippocampal HT22 Cell Line
- Authors
- Lee, Seoung Rak; Song, Ji Hoon; Song, Jae-Hyoung; Ko, Hyun-Jeong; Baek, Ji Yun; Tuy An Trinh; Beemelmanns, Christine; Yamabe, Noriko; Kim, Ki Hyun
- Issue Date
- Sep-2018
- Publisher
- MDPI
- Keywords
- fungus-growing termite; Streptomyces sp.; isoflavonoid; neuroprotective effect; glutamate
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.19, no.9
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- Volume
- 19
- Number
- 9
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3426
- DOI
- 10.3390/ijms19092640
- ISSN
- 1422-0067
- Abstract
- Insect-associated bacteria have been recognized as a very promising natural resource for discovering bioactive secondary metabolites with diverse pharmacological effects. One new isoflavonoid glycoside, termisoflavone D (1), together with seven known isoflavonoids (2-8), were identified from MeOH extracts of the fungus-growing termite-associated Streptomyces sp. RB1. The chemical structure of the new compound 1 was elucidated using comprehensive spectroscopic methods including 1D and 2D NMR, along with LC/MS analysis. The existence of two rhamnose moieties in 1 was determined with comparative NMR analysis, and the absolute configuration was elucidated using chemical reactions. The neuroprotective activities of compounds 1-8 were thoroughly investigated using the murine hippocampal HT22 cell line. Compound 5 prevented glutamate-induced HT22 cell death by blocking intracellular reactive oxygen species (ROS) accumulation. The present study provides the first experimental evidence for the potential use of isoflavonoids from termite-associated bacteria as lead compounds that can prevent neuronal damage induced by glutamate.
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