Bioactivity evaluations of betulin identified from the bark of Betula platyphylla var. japonica for cancer therapy
- Authors
- So, Hae Min; Eom, Hee Jeong; Lee, Dahae; Kim, Sil; Kang, Ki Sung; Lee, Il Kyun; Baek, Kwan-Hyuck; Park, Jun Yeon; Kim, Ki Hyun
- Issue Date
- Aug-2018
- Publisher
- PHARMACEUTICAL SOC KOREA
- Keywords
- Betula platyphylla var. japonica; Betulin; Cytotoxicity; Nephrotoxicity; Anti-gastritis
- Citation
- ARCHIVES OF PHARMACAL RESEARCH, v.41, no.8, pp.815 - 822
- Journal Title
- ARCHIVES OF PHARMACAL RESEARCH
- Volume
- 41
- Number
- 8
- Start Page
- 815
- End Page
- 822
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3544
- DOI
- 10.1007/s12272-018-1064-9
- ISSN
- 0253-6269
- Abstract
- Identification of bioactive natural products with anticancer activity as well as alleviating effects on chemotherapy-induced side effects has significant implications for cancer treatment. Betula platyphylla var. japonica, commonly known as Asian white birch, has been used in Chinese traditional medicine for a variety of purposes. In this study, the medicinal properties of betulin from B. platyphylla var. japonica useful for cancer management were investigated. LC/MS analysis revealed that betulin is a main chemical component of the EtOH extract of B. platyphylla var. japonica bark, and betulin was isolated from EtOH extract using an LC/MS-guided isolation method. Its structure was identified with H-1 and C-13 NMR spectroscopic data and LC/MS analysis and then compared to the previously reported spectroscopic and physical data. We first verified the cytotoxicity of betulin against three human lung adenocarcinoma cell lines, A549, H1264, and Calu-6, with IC50 values ranging from 18.7 to 39.6 mu M. Regarding alleviation of side effects associated with anticancer chemotherapy, betulin ameliorated cisplatin-induced renal cell damage to 80% of the control value from the concentration of 5 mu M. In addition, betulin showed anti-gastritis activity against ethanol-induced gastric damage in rats and notably reduced the gastric damage index compared to control in a concentration-dependent manner. These findings provide the first experimental evidence for potential use of B. platyphylla var. japonica as a functional food for cancer treatment that simultaneously alleviates the side effects of chemotherapy.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 한의과대학 > 한의예과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3544)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.