Determination of isoorientin levels in rat plasma after oral administration of Vaccinum bracteatum Thunb. methanol extract by high-performance liquid chromatography-tandem mass spectrometry
- Authors
- Kim, Min-Ji; Kwon, Seung-Hwan; Jang, Choon-Gon; Maeng, Han-Joo
- Issue Date
- Jun-2018
- Publisher
- WILEY
- Keywords
- isoorientin; LC-MS/MS; pharmacokinetics; plasma; rat; Vaccinium bracteatum Thunb; methanol extract
- Citation
- BIOMEDICAL CHROMATOGRAPHY, v.32, no.6
- Journal Title
- BIOMEDICAL CHROMATOGRAPHY
- Volume
- 32
- Number
- 6
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3694
- DOI
- 10.1002/bmc.4188
- ISSN
- 0269-3879
- Abstract
- A simple, sensitive and rapid liquid chromatography tandem mass spectrometry method (LC-MS/MS) was developed and validated for the determination of plasma isoorientin levels in rats. After simple protein precipitation using methanol, chromatographic analysis was performed using a Synergi 4 polar-RP 80A column (150 x 2.0mm, 4m) under isocratic conditions and a mobile phase consisting of 0.1% formic acid in water and methanol (80:20, v/v) at a flow rate of 0.2mL/min. In positive electrospray ionization mode, the protonated precursor and product ion transitions of isoorientin (m/z 449.0 299.1) and of puerarin (the internal standard; m/z 417.1 297.1) were acquired by multiple reaction monitoring. Calibration curves obtained for plasma showed good linearity over the concentration range 1-1000ng/mL. The lower limit of quantification was 1ng/mL. Intra- and inter-day precisions were within 8.8% relative standard deviation. Accuracies ranged from 92.1 and 109.7%. The isoorientin stability in rat plasma under typical handling/storage conditions also found to be acceptable. The developed method was applied successfully to a pharmacokinetic study of isoorientin orally administered as the methanol extract of Vaccinium bracteatum Thunb. or administered as pure isoorientin.
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