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Free Radical Initiated Peptide Sequencing Using MALDI-TOF/TOF Mass Spectrometry
- Issue Date
- Jun-2018
- Publisher
- KOREAN SOC MASS SPECTROMETRY
- Keywords
- FRIPS; MALDI-TOF/TOF; mass spectrometry; peptides; radical ion; radical-driven fragmentation
- Citation
- MASS SPECTROMETRY LETTERS, v.9, no.2, pp.56 - 60
- Journal Title
- MASS SPECTROMETRY LETTERS
- Volume
- 9
- Number
- 2
- Start Page
- 56
- End Page
- 60
- ISSN
- 2233-4203
- Abstract
- In this study, matrix-assisted laser desorption/ionization (MALDI) was applied to the TEMPO-assisted FRIPS for the first time. We found that 3-HPA is the optimal matrix for the analysis of p-TEMPO-Bz-Sc-peptides, which gives minimal precursor fragmentations. MALDI-TOF/TOF experiments on p-TEMPO-Bz-Sc-peptides yielded mainly [a(n)+H](+), [z(n)+H](+), and [y(n)](+)-type products, indicating that radical-driven peptide fragmentation occurs in MALDI-TOF/TOF-MS.
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Related Researcher
- Han, Sang Yun
- BioNano Technology (Department of Chemistry)