Optimization of Cytokine Milieu to Reproduce Atopic Dermatitis-related Gene Expression in HaCaT Keratinocyte Cell Line
- Authors
- Kim, Hee Joo; Baek, Jinok; Lee, Jong Rok; Roh, Joo Young; Jung, YunJae
- Issue Date
- Apr-2018
- Publisher
- KOREA ASSOC IMMUNOLOGISTS
- Keywords
- Atopic dermatitis; Cytokine; In vitro stimulation
- Citation
- IMMUNE NETWORK, v.18, no.2
- Journal Title
- IMMUNE NETWORK
- Volume
- 18
- Number
- 2
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3915
- DOI
- 10.4110/in.2018.18.e9
- ISSN
- 1598-2629
- Abstract
- Although atopic dermatitis (AD) is characterized by cytokine production predominantly mediated by T helper (Th) 2 cells, AD pathogenesis also involves innate immune and Th1 cells. To optimize the cytokine milieu required for accurate reproduction of AD-related gene expression profile in vitro, we evaluated the expression pattern of CCL22, CCL17, IL5, IL13, IL33, IL25, TSLP, FLG, and LOR in human lesional AD skin and cytokine-stimulated HaCaT cells. An increase in Th2 mediators (IL5, IL13, CCL22, CCL17, IL25, IL33, and TSLP) and a decrease in genes related to cornified cell envelope (filaggrin and loricrin) were observed in human AD lesions. Innate (tumor necrosis factor-alpha) and/or Th1/Th2 adaptive cytokines (interferon-gamma/IL-4) were required for inducing these inflammatory changes in HaCaT cells, implying that a complex network of innate, Th1, and Th2 cytokines drives AD-like changes. Therefore, stimulation with various combinations of cytokines, beyond Th2 polarization, is necessary when HaCaT cell line is used to study genetic changes implicated in AD pathogenesis.
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