만성 저온 스트레스 동물모델에서의 黃連의 항염증 효능 연구Anti-inflammatory effects of Coptidis Rhizoma in chronic cold stress-exposed mice
- Other Titles
- Anti-inflammatory effects of Coptidis Rhizoma in chronic cold stress-exposed mice
- Authors
- 최진규; 허유진; 이원일; 김윤경; 이태희; 오명숙
- Issue Date
- 2018
- Publisher
- 대한본초학회
- Keywords
- Coptidis Rhizoma; chronic cold stress; inflammation
- Citation
- 대한본초학회지, v.33, no.6, pp.35 - 42
- Journal Title
- 대한본초학회지
- Volume
- 33
- Number
- 6
- Start Page
- 35
- End Page
- 42
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/4637
- DOI
- 10.6116/kjh.2018.33.6.35
- ISSN
- 1229-1765
- Abstract
- Objectives : The aim of this study was to investigate whether the extract of Coptidis Rhizoma inhibits inflammation in chronic cold stress (CCS)-exposed mice or not.
Methods : Coptidis Rhizoma extract (CRE) was made by reflux with distilled water. Male ICR mice (7 weeks old) were divided randomly into 5 groups: (1) control, (2) CCS, (3) CCS+CRE 100 ㎎/㎏, (4) CCS+CRE 300 ㎎/㎏, (5) CCS+CRE 1,000 ㎎/㎏ groups. Mice were orally administered once a day for 14 days starting from 1 day before CCS. Group (2)-(5) were exposed to CCS conditions that maintained at 4℃ for 2 h once a day for 14 days. The levels of serum cortisol and hypothalamic prostaglandin E1 (PGE1) and PGE2 were measured by enzyme-linked immunosorbent assay kit. The expression levels of several pro-inflammatory factors like heat shock protein 70 (HSP70), c-fos, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) were measured by western blot analysis in mouse hypothalamus.
Results : Oral administration of CRE 1,000 ㎎/㎏ significantly suppressed the increase of serum cortisol levels in mice exposed to CCS. CCS-exposed mice had significantly increased the expression of HSP70, c-fos, and NF-kB in hypothalamus, while CRE treatment significantly attenuated the elevation of these pro-inflammatory factors. The ratio of PGE2/PGE1 was also higher in CCS-exposed mice than control group. CRE treatment significantly reduced the increase of PGE2/PGE1 ratio induced by CCS.
Conclusion : These findings suggest that Coptidis Rhizoma may work as a potential agent to modulate inflammatory responses under the condition of cold adaptation formed by CCS.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 한의과대학 > 한의예과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/4637)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.