梔子의 Apoptosis유발 억제효과Inhibitory Effects of Gardeniae Fructus on Apoptosis induced by 4-HNE in PC-12 Cell
- Other Titles
- Inhibitory Effects of Gardeniae Fructus on Apoptosis induced by 4-HNE in PC-12 Cell
- Authors
- 한영수; 이태희; 김연섭
- Issue Date
- Aug-2018
- Publisher
- 대한한의학방제학회
- Keywords
- Gardeniae Fructus; 4-HNE; Bax; Bcl-2; Caspase-3; TNF-α
- Citation
- 대한한의학 방제학회지, v.26, no.3, pp.195 - 205
- Journal Title
- 대한한의학 방제학회지
- Volume
- 26
- Number
- 3
- Start Page
- 195
- End Page
- 205
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/4840
- DOI
- 10.14374/HFS.2018.26.3.195
- ISSN
- 1229-1218
- Abstract
- Objectives : The purpose of this study was to observe the effects of Gardeniae Fructus on 4-HNE-induced apoptosis in PC-12 cell.
Methods : A MTT assay was conducted to observe the cytotoxicity of Gardeniae Fructus on the PC-12 cell viability and the cytoprotective effects of Gardeniae Fructus on PC-12 cell against oxidative stress caused by 4-HNE. And western blot was conducted to observe the expression of Bax, Bcl-2, Caspase-3, TNF-α proteins which are involved in intrinsic and extrinsic apoptosis pathway.
Results : 25, 50, 100, 200 and 400 ㎍/㎖ of Gardeniae Fructus water extract had no cytotoxicity on PC-12 cell.
200 ㎍/㎖ of Gardeniae Fructus water extract had significant cytoprotective effect on PC-12 cell against oxidative stress caused by 4-HNE.
The expression of Bax protein in 50, 100 and 200 ㎍/㎖ of Gardeniae Fructus was significantly decreased in PC-12 cell.
The expression of Bcl-2 protein in 200 ㎍/㎖ of Gardeniae Fructus was significantly increased in PC-12 cell.
The expression of Caspase-3 protein in 100 and 200 ㎍/㎖ of Gardeniae Fructus was significantly decreased in PC-12 cell.
The expression of TNF-α protein in 50 ㎍/㎖ of Gardeniae Fructus was significantly decreased in PC-12 cell.
Conclusions : These results suggest that Gardeniae Fructus water extract is effective to protect PC-12 cell from 4-HNE induced apoptosis.
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