Corneal Wound Healing Effects of Mesenchymal Stem Cell Secretome Delivered Within a Viscoelastic Gel Carrier
- Authors
- Fernandes-Cunha, Gabriella Maria; Na, Kyung-Sun; Putra, Ilham; Lee, Hyun Jong; Hull, Sarah; Cheng, Yu-Chia; Blanco, Ignacio Jesus; Eslani, Medi; Djalilian, Ali R.; Myung, David
- Issue Date
- May-2019
- Publisher
- WILEY
- Keywords
- Cellular proliferation; Chondroitin sulfate; Cornea; Mesenchymal stem cells
- Citation
- STEM CELLS TRANSLATIONAL MEDICINE, v.8, no.5, pp.478 - 489
- Journal Title
- STEM CELLS TRANSLATIONAL MEDICINE
- Volume
- 8
- Number
- 5
- Start Page
- 478
- End Page
- 489
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/53886
- DOI
- 10.1002/sctm.18-0178
- ISSN
- 2157-6564
- Abstract
- Severe corneal injuries often result in permanent vision loss and remain a clinical challenge. Human bone marrow-derived mesenchymal stem cells (MSCs) and their secreted factors (secretome) have been studied for their antiscarring, anti-inflammatory, and antiangiogeneic properties. We aimed to deliver lyophilized MSC secretome (MSC-S) within a viscoelastic gel composed of hyaluronic acid (HA) and chondroitin sulfate (CS) as a way to enhance corneal re-epithelialization and reduce complications after mechanical and chemical injuries of the cornea. We hypothesized that delivering MSC-S within HA/CS would have improved wound healing effects compared the with either MSC-S or HA/CS alone. The results showed that a once-daily application of MSC-S in HA/CS enhances epithelial cell proliferation and wound healing after injury to the cornea. It also reduced scar formation, neovascularization, and hemorrhage after alkaline corneal burns. We found that combining MSC-S and HA/CS increased the expression of CD44 receptors colocalized with HA, suggesting that the observed therapeutic effects between the MSC-S and HA/CS are in part mediated by CD44 receptor upregulation and activation by HA. The results from this study demonstrate a reproducible and efficient approach for delivering the MSC-S to the ocular surface for treatment of severe corneal injuries. Stem Cells Translational Medicine 2019;8:478-489
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