HPLC-UV analysis of sample preparation influence on flavonoid yield from Cirsium japonicum var. maackii

Abstract

This study was conducted to optimize the extraction conditions of flavonoids from Cirsium japonicum var. maackii (ICF-1). The effects of sample material ratio, solvent concentration, extraction time, solid-to-solvent ratio, and number of extractions on flavonoid extraction efficiency were analyzed. Three flavonoids were specifically investigated: cirsimarin (1), hispidulin (2), and cirsimaritin (3). In single-factor experiments, each variable had a significant effect on the determination of content of compounds 1-3. The optimal conditions for extraction were found to be: mass, 15 g; ratio of spring and fall leaves, 4:1; extraction solvent, 70% ethanol; extraction time, 4 h; solid-to-solvent ratio, 1:20; and number of extractions, 1. The results of the study were used to maximize the potential of ICF-1 samples and optimize the efficiency of the extraction process.