HPLC-UV analysis of sample preparation influence on flavonoid yield from Cirsium japonicum var. maackii
- Authors
- Rodriguez, Joyce P.; Lee, Jaemin; Park, Jun Yeon; Kang, Ki Sung; Hahm, Dae-Hyun; Lee, Sang Cheon; Lee, Sanghyun
- Issue Date
- Oct-2017
- Publisher
- KOREAN SOC APPLIED BIOLOGICAL CHEMISTRY
- Keywords
- Cirsimarin; Cirsimaritin; Cirsium japonicum var. maackii; High-performance liquid chromatography-UV; Hispidulin
- Citation
- APPLIED BIOLOGICAL CHEMISTRY, v.60, no.5, pp.519 - 525
- Journal Title
- APPLIED BIOLOGICAL CHEMISTRY
- Volume
- 60
- Number
- 5
- Start Page
- 519
- End Page
- 525
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/5669
- DOI
- 10.1007/s13765-017-0306-8
- ISSN
- 2468-0834
- Abstract
- This study was conducted to optimize the extraction conditions of flavonoids from Cirsium japonicum var. maackii (ICF-1). The effects of sample material ratio, solvent concentration, extraction time, solid-to-solvent ratio, and number of extractions on flavonoid extraction efficiency were analyzed. Three flavonoids were specifically investigated: cirsimarin (1), hispidulin (2), and cirsimaritin (3). In single-factor experiments, each variable had a significant effect on the determination of content of compounds 1-3. The optimal conditions for extraction were found to be: mass, 15 g; ratio of spring and fall leaves, 4:1; extraction solvent, 70% ethanol; extraction time, 4 h; solid-to-solvent ratio, 1:20; and number of extractions, 1. The results of the study were used to maximize the potential of ICF-1 samples and optimize the efficiency of the extraction process.
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