Physical structure and absorption properties of tailor-made porous starch granules produced by selected amylolytic enzymes
- Authors
- Jung, Yi-seul; Lee, Byung-Hoo; Yoo, Sang-Ho
- Issue Date
- 20-Jul-2017
- Publisher
- PUBLIC LIBRARY SCIENCE
- Citation
- PLOS ONE, v.12, no.7
- Journal Title
- PLOS ONE
- Volume
- 12
- Number
- 7
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/5913
- DOI
- 10.1371/journal.pone.0181372
- ISSN
- 1932-6203
- Abstract
- Porous starch granules (PSGs) with various pores and cavity sizes were prepared by amylolysis enzymes. The greatest hydrolysis rate on corn starch granule was observed with alpha-amylase, followed by gluco- and beta-amylases. Temperature increase enhanced glucoamylase reaction rate more drastically than other enzyme treatments. Final hydrolysis level with glucoamylase reached to 66.9%, close to 67.5% of alpha-amylolysis. The alpha-amylase-treated PSGs displayed the greatest pore size and ratio of cavity-to-granule diameters. Gelatinization onset temperatures of PSGs increased to 72.1 (alpha-), 68.7 (beta-), and 68.1 degrees C (gluco-amylolysis) after 8 h; enthalpy changes of beta- and gluco-amylase-treated PSGs increased to 13.4, and 13.1 J/g but alpha-amylase-treated one showed slightly reduced value of 8.5 J/g. Water holding capacities of PSGs were 209.7 (alpha-), 94.6 (beta-), and 133.8% (gluco-amylolysis), and the untreated control had 89.1%; oil holding capacities of them showed 304.5, 182.7, and 211.5%, respectively, while the untreated control had 161.8%. Thus, enzyme types and their reaction conditions can be applied to generate desirable cavity and pore sizes in starch granules. This biocatalytic approach could contribute to develop tailor-made PSGs with distinct internal structure for specific uses in wide range of food, pharmaceutical and other industrial applications.
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