An ultra-sensitive biophysical risk assessment of light effect on skin cells
- Authors
- Bennet, Devasier; Viswanath, Buddolla; Kim, Sanghyo; An, Jeong Ho
- Issue Date
- 18-Jul-2017
- Publisher
- IMPACT JOURNALS LLC
- Keywords
- biophysics of organization; biomechanical changes; color light radiation; extracellular matrix degeneration; AFM and ECIS based analysis
- Citation
- ONCOTARGET, v.8, no.29, pp.47861 - 47875
- Journal Title
- ONCOTARGET
- Volume
- 8
- Number
- 29
- Start Page
- 47861
- End Page
- 47875
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/5915
- DOI
- 10.18632/oncotarget.18136
- ISSN
- 1949-2553
- Abstract
- The aim of this study was to analyze photo-dynamic and photo-pathology changes of different color light radiations on human adult skin cells. We used a real-time biophysical and biomechanics monitoring system for light-induced cellular changes in an in vitro model to find mechanisms of the initial and continuous degenerative process. Cells were exposed to intermittent, mild and intense (1-180 min) light with On/Off cycles, using blue, green, red and white light. Cellular ultrastructural changes, damages, and ECM impair function were evaluated by up/downregulation of biophysical, biomechanical and biochemical properties. All cells exposed to different color light radiation showed significant changes in a time-dependent manner. Particularly, cell growth, stiffness, roughness, cytoskeletal integrity and ECM proteins of the human dermal fibroblasts-adult (HDF-a) cells showed highest alteration, followed by human epidermal keratinocytes-adult (HEK-a) cells and human epidermal melanocytes-adult (HEM-a) cells. Such changes might impede the normal cellular functions. Overall, the obtained results identify a new insight that may contribute to premature aging, and causes it to look aged in younger people. Moreover, these results advance our understanding of the different color light-induced degenerative process and help the development of new therapeutic strategies.
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