Effects of Prunus mume Siebold & Zucc. in the pacemaking activity of interstitial cells of Cajal in murine small intestine
- Authors
- Lee, Sang Weon; Kim, Sung Jin; Kim, Hyungwoo; Yang, Dongki; Kim, Hyun Jung; Kim, Byung Joo
- Issue Date
- Jan-2017
- Publisher
- SPANDIDOS PUBL LTD
- Keywords
- interstitial cells of Cajal; plum; Prunus mume Siebold & Zucc.; gastrointestinal tract
- Citation
- EXPERIMENTAL AND THERAPEUTIC MEDICINE, v.13, no.1, pp.327 - 334
- Journal Title
- EXPERIMENTAL AND THERAPEUTIC MEDICINE
- Volume
- 13
- Number
- 1
- Start Page
- 327
- End Page
- 334
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/6525
- DOI
- 10.3892/etm.2016.3963
- ISSN
- 1792-0981
- Abstract
- Interstitial cells of Cajal (ICCs) function as pacemaker cells in the gastrointestinal (GI) tract and therefore, serve an important role in regulating GI motility. The effects of a species of plum (Prunus mume Siebold & Zucc.) on cultured ICC cluster-induced pacemaker potentials in the mouse small intestine were investigated, and the effects of a methanolic extract of Prunus mume (m-PM) on ICC pacemaker activities were examined using the whole-cell patch-clamp technique. ICC pacemaker membrane potentials were depolarized by m-PM in a concentration dependent manner in current clamp mode. 4-Diphenylacetoxy-N-methyl-piperidine methiodide, which is a muscarinic 3 (M-3) receptor antagonist, was able to block m-PM-induced pacemaker potential increases, whereas methoctramine, which is a muscarinic 2 (M-2) receptor antagonist, was not. When 1 mM guanosine diphosphate (3-5 was present in the pipette solution, m-PM induced slight pacemaker depolarization. Following pretreatment in bath solution of Ca2+-free solution or a Ca'+-ATPase inhibitor in endoplasmic reticulum, the pacemaker currents were inhibited. Furthermore, pretreatment with PD98059, SB203580 or SP600125, which is a c-jun NH2-terminal kinase inhibitor, blocked m-PM-induced ICC potential depolarization. Furthermore, m-PM inhibited transient receptor potential melastatin (TRPM) 7 channels, but did not affect Ca2+-activated Cl- channels. These results suggest that m-PM is able to modulate pacemaker potentials through the muscarinic M-3 receptor, via G-protein and external and internal Ca2+, in a mitogen-activated protein kinase and TRPM7-dependent manner. Therefore, m-PM may provide a basis for the development of a novel gastroprokinetic agent.
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