Naringenin causes ASK1-induced apoptosis via reactive oxygen species in human pancreatic cancer cells
- Authors
- Park, Hyun Jin; Choi, Yong Jun; Lee, Jeong Hyun; Nam, Myeong Jin
- Issue Date
- Jan-2017
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Naringenin; Pancreatic cancer; Peroxiredoxin-1; Reactive oxygen species; Apoptosis signal-regulation kinase 1
- Citation
- FOOD AND CHEMICAL TOXICOLOGY, v.99, pp.1 - 8
- Journal Title
- FOOD AND CHEMICAL TOXICOLOGY
- Volume
- 99
- Start Page
- 1
- End Page
- 8
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/6574
- DOI
- 10.1016/j.fct.2016.11.008
- ISSN
- 0278-6915
- Abstract
- Naringenin, one of the most abundant flavonoids in natural citrus fruits, has been investigated for its ability to inhibit growth of breast, colon, gastric and prostate cancer cells. However, naringenin-induced cell death in pancreatic cancer is not well understood. Therefore, we analyzed the naringenin-induced apoptosis mechanism using human pancreatic cancer SNU-213 cells. Annexin V+/PI + marked cells increased from 5.10% to 8.29%, 25.06% and 35.31% in response to treatment with 200, 400, and 600 mu M naringenin, respectively. Two-dimensional electrophoresis to identify possible target-related proteins of naringenin-induced apoptosis revealed seven proteins. Among these, the expression of peroxiredoxin-1 (Prdx-1), which modulates redox homeostasis of cells, was decreased. To obtain a broad understanding of the interactive mechanism of naringenin and Prdx-1, we observed changes in reactive oxygen species (ROS) in naringenin-treated SNU-213 cells. The ROS levels were 130.02 +/- 20.21%, 182.04 +/- 5.39%, and 237.21 +/- 12.71% in response to 200, 400, and 600 mu M naringenin treatment, respectively. Increases in ROS were followed by up-regulation of apoptosis signal-regulation kinase 1 (ASK1). Moreover, the JNK, p38 and p53 proteins were upregulated. Overall, the results of this study suggest that naringenin causes ASK1-induced apoptosis mediated by ROS. (C) 2016 Elsevier Ltd. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 바이오나노대학 > 생명과학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/6574)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.