Leuconostoc lactis CCK940의 Glucansucrase 활성에 의한 올리고당 생산 최적화Oligosaccharide Production by Leuconostoc lactis CCK940 Which Has Glucansucrase Activity
- Other Titles
- Oligosaccharide Production by Leuconostoc lactis CCK940 Which Has Glucansucrase Activity
- Authors
- 이설희; 박영서
- Issue Date
- 2017
- Publisher
- 한국산업식품공학회
- Keywords
- lactic acid bacteria; oligosaccharide; Leuconostoc lactis; optimization; glucansucrase
- Citation
- 산업식품공학, v.21, no.4, pp.383 - 390
- Journal Title
- 산업식품공학
- Volume
- 21
- Number
- 4
- Start Page
- 383
- End Page
- 390
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/6750
- ISSN
- 1226-4768
- Abstract
- Glucansucrase is an enzyme classified as a glycoside hydrolase (GH) 70 family, which catalyzes the synthesis of glucooligosaccharides with a low molecular weight using sucrose as a donor of D-glucopyranose and maltose as a carbohydrate acceptor. In this study, glucansucrase-producing lactic acid bacteria strain was isolated from the fermented foods collected in traditional markets, and the optimum conditions for the oligosaccharide production were investigated. The strain CCK940 isolated from Chinese cabbage kimchi was selected as an oligosaccharide-producing strain due to its high glucansucrase activity, with 918.2 mU/mL, and identified as Leuconostoc lactis. The optimum conditions for the production of oligosaccharides using Leu. lactis CCK940 were to adjust the initial pH to 6.0, add 5% (w/v) sucrose and 10% (w/v) maltose as a donor and acceptor molecules, respectively, and feed 5% (w/v) sucrose at 4 and 8 h of cultivation. When Leu. lactis CCK940 was cultured for 12 h at optimum conditions, at least four oligosaccharides with a polymerization degree of 2-4 were produced.
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