Identification and Characterization of a Splicing Variant in the 5 ' UTR of the Human TLR5 Gene
- Authors
- Hoang, Thi Xoan; Duong, Cao Nguyen; Kim, Jae Young
- Issue Date
- Aug-2017
- Publisher
- HINDAWI LTD
- Citation
- BIOMED RESEARCH INTERNATIONAL
- Journal Title
- BIOMED RESEARCH INTERNATIONAL
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/7485
- DOI
- 10.1155/2017/8727434
- ISSN
- 2314-6133
- Abstract
- Toll-like receptors (TLRs) are essential components of the innate immune system. TLR5 is the receptor for flagellin, the principal protein component of bacterial flagella. The TLR5 gene has 6 exons. In an RT-PCR analysis, we found long TLR5 transcripts, in addition to those of the expected size (short TLR5 transcripts). A sequence analysis revealed that the long TLR5 transcripts contain a new exon of 94 nucleotides located between previously reported exons IV and V in the 5' untranslated region (5.. UTR). A realtime PCR analysis of the two alternatively spliced variants in various cell lines showed that the long TLR5 transcripts are abundantly expressed in nonimmune cells. The ratios of long/short transcripts in human nonimmune cell lines, such as A549, T98G, HaCaT, H460, HEK-293, and Caco-2 cells, and primary mesenchymal stemcells were in the range of 1.25 to 4.31. In contrast, those of human monocytic THP-1 and U937 cells and E6.1 T cells and Ramos B cells were around 0.9. These ratios in humanmonocytic THP-1 cells were decreased by treatment with IFN-gamma in a concentration-dependent manner. Based on our findings, we suggest that the newly found long TLR5 transcripts may be involved in the negative regulation of TLR5 expression and function.
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