Naphthoquinones from Handroanthus impetiginosus promote skin wound healing through Sirt3 regulation
- Authors
- Ahmad, Fayyaz; Bibi, Shaheen; Kang, Mincheol; Anees, Mariam; Ansar, Muhammad; Alam, Muhammad Rizwan; Kim, Sun Yeou; Wahedi, Hussain Mustatab
- Issue Date
- Sep-2020
- Publisher
- University of Medical Sciences
- Keywords
- Beta-Lapachone; Dermatology; Inflammation; Regeneration; Tabebuia
- Citation
- Iranian Journal of Basic Medical Sciences, v.23, no.9, pp.1139 - 1145
- Journal Title
- Iranian Journal of Basic Medical Sciences
- Volume
- 23
- Number
- 9
- Start Page
- 1139
- End Page
- 1145
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/76299
- DOI
- 10.22038/ijbms.2020.43706.10275
- ISSN
- 2008-3866
- Abstract
- Objective(s): Lapachone is a natural naphthoquinone-derived compound found in Tabebuia avellanedae. It is well-known for its analgesic, anti-inflammatory, anti -microbial, diuretic, and anti cancerous effects. However, the wound-healing effects of this compound are not known yet. The aim of this study was to investigate the wound healing activity of naphthoquinones (alpha-lapachone and beta-lapachone) from Handroanthus impetiginosus. Materials and Methods: Expression of Sirt3, migration-related proteins (Racl, Cdc42, alpha-Pak) and angiogenesis-related protein of vascular endothelial growth factor (VEGF) was monitored using western blot analysis. Blood vessel formation and tissue development were monitored by angiogenesis assay and hematoxylin & eosin (H & E) staining, respectively on mouse skin tissue samples. Both alpha-lapachone and beta-lapachone increased Sirt3 expression in vivo, but only beta-lapachone increased Sirt3 expression in vitro. Results: Both the compounds accelerated wound healing in cultured skin cells as well as mouse skin; however, beta-lapachone was more effective at lower concentrations. Both of the compounds increased the expression of migration -related proteins both in vitro and in vivo. Similarly, alpha-lapachone and beta-lapachone increased VEGF expression, tissue development and blood vessel formation in mouse skin. Conclusion: These findings indicated that alpha-lapachone and beta-lapachone are novel Sirt3 activators, and Sirt3 has a role in wound healing. Thus, Sirt3 and its regulators come out as a novel target and potential drug candidates, respectively in the important field of cutaneous wound healing.
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