Detailed Information

Cited 48 time in webofscience Cited 49 time in scopus
Metadata Downloads

Cold atmospheric plasma generated reactive species aided inhibitory effects on human melanoma cells: an in vitro and in silico study

Authors
Yadav, Dharmendra KumarAdhikari, ManishKumar, SurendraGhimire, BhagirathHan, IhnKim, Mi-HyunChoi, Eun-Ha
Issue Date
Feb-2020
Publisher
NATURE PUBLISHING GROUP
Citation
SCIENTIFIC REPORTS, v.10, no.1
Journal Title
SCIENTIFIC REPORTS
Volume
10
Number
1
URI
https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/78191
DOI
10.1038/s41598-020-60356-0
ISSN
2045-2322
Abstract
Malignant melanoma is considered to be a heterogeneous disease that arises from altered genes and transformed melanocytes. In this study, special softjet cold atmospheric plasma was used to treat three different human melanoma cells using air and N-2 gases to check the anti-melanoma activity. The physical effects by plasma revealed an increase in the temperature with the gradual reduction in pH at 60 sec, 180 sec and 300 sec air and N-2 plasma treatment. Cellular toxicity revealed a decreased in cell survival (similar to 50% cell survival using air gas and <similar to 60% cell survival using N-2 gas at 60 sec plasma treatment in G-361 cells). Gene analysis by q-PCR revealed that 3 min and 5 min air and N-2 plasma treatment activated apoptotic pathways by triggering apoptotic genes in all three melanoma cell lines. The apoptosis was confirmed by DAPI staining and its related pathways were further explored according to protein-protein docking, and their probable activation mechanism was revealed. The pathways highlighted that activation of apoptosis which leads to cellular cascades and hence stimulation ASK1 (docking method) revealed that softjet plasma can be an effective modality for human melanoma treatment.
Files in This Item
There are no files associated with this item.
Appears in
Collections
약학대학 > 약학과 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Yadav, Dharmendra Kumar photo

Yadav, Dharmendra Kumar
Pharmacy (Department of Biologics)
Read more

Altmetrics

Total Views & Downloads

BROWSE