저온 노출된 혈관 세포 내 RhoA 단백질 활성화에 대한 십전대보탕의 억제 효과Mitigative Effect of Sipjeondaebo-tang on RhoA Activation in Cold-Exposed Vascular Cells
- Other Titles
- Mitigative Effect of Sipjeondaebo-tang on RhoA Activation in Cold-Exposed Vascular Cells
- Authors
- 이강욱; 김명선; 김윤경; 황현하; 고호연; 선승호; 최유경; 양승보; 송윤경; 전찬용; 고성규
- Issue Date
- Sep-2020
- Publisher
- 대한한방내과학회
- Keywords
- Raynaud’s phenomenon; cold; rhoA; endothelin-1; nitric oxide; Sipjeondaebo-tang
- Citation
- 대한한방내과학회지, v.41, no.4, pp.553 - 562
- Journal Title
- 대한한방내과학회지
- Volume
- 41
- Number
- 4
- Start Page
- 553
- End Page
- 562
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/78320
- DOI
- 10.22246/jikm.2020.41.4.553
- ISSN
- 1226-9174
- Abstract
- Objective: To investigate the vasodilatory effect of Sipjeondaebo-tang by inhibiting RhoA activity in vascular cells during cold exposure.
Methods: Human vascular endothelial cells and pericytes were pretreated with Sipjeondaebo-tang for 30 min, followed by incubation at 37 ℃ (control) or 25 ℃ (cold exposure) for 30 min. Activation of endothelin-1-mediated RhoA in pericytes was assessed by pretreating the cells with Sipjeondaebo-tang for 30 min, followed by incubation with endothelin-1 at 37 ℃ for 30 min. Western blotting was performed to measure the expression of active RhoA. Endothelin-1 and nitric oxide release from endothelial cells was examined with enzyme-linked immunosorbent assay kits. The formation of stress fibers and focal adhesion complexes was analyzed by immunocytochemistry.
Results: Cold treatment activated RhoA in both pericytes and vascular endothelial cells, whereas Sipjeondaebo-tang treatment inhibited this activation. Sipjeondaebo-tang treatment also reversed the cold-mediated production of endothelin-1 and nitric oxide. Cold exposure promoted the formation of stress fibers and focal adhesion complexes by increasing the expression of phospho-focal adhesion complex kinase, whereas Sipjeondaebo-tang treatment suppressed this response.
Conclusions: These findings suggested that Sipjeondaebo-tang inhibits cold-induced RhoA activation and its related pathway components, including endothelin-1 and nitric oxide, in vascular cells. Therefore, Sipjeondaebo-tang could be beneficial for the treatment of Raynaud’s phenomenon.
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