Label-free liquid crystal-based biosensor for detection of dopamine using DNA aptamer as a recognition probe
- Authors
- Nguyen, D.K.; Jang, C.-H.
- Issue Date
- Sep-2020
- Publisher
- Academic Press Inc.
- Keywords
- Dopamine; Dopamine-binding aptamer; LC-Based aptasensors; Liquid crystals
- Citation
- Analytical Biochemistry, v.605
- Journal Title
- Analytical Biochemistry
- Volume
- 605
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/78411
- DOI
- 10.1016/j.ab.2020.113807
- ISSN
- 0003-2697
- Abstract
- We present a label-free liquid crystal-based biosensor for the detection of dopamine (DA) in aqueous solutions using dopamine-binding aptamers (DBA) as recognition elements. In this system, the dimethyloctadecyl [3-(trimethoxysilyl) propyl] ammonium chloride (DMOAP) self-assembled monolayers immobilized on glass slides support the long alkyl chains that keep the liquid crystal (LC) molecules in a homeotropic orientation. Glutaraldehyde (GA) is used as a cross-linker to immobilize DBA onto the surface of glass slides. The specific binding of DA and DBA disrupts the homeotropic orientation of LCs, thereby inducing a change in the orientation from homeotropic to a random alignment. This orientation change can be converted and visualized simply as a transition from a dark optical LC image to a brighter image under a polarized optical microscope (POM), enabling the detection of DA. The developed LC-based aptasensor shows a good linear optical response towards DA in the very wide range of 1 pM–10 μM (0.19 pg/mL to 1.9 μg/mL) and has a very low detection limit of 10 pM (∼1.9 pg/mL). The biosensor also exhibited satisfactory selectivity and could be successfully applied to detect DA in human urine. The proposed LC-based aptamer sensing method offers a simple, rapid, highly sensitive and selective, and a label-free method for the analysis of DA in real clinical samples. © 2020 Elsevier Inc.
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