Analysis and anticancer effects of active compounds from Spatholobi caulis in human breast cancer cells
- Authors
- Phung, H.M.; Lee, H.; Lee, S.; Jang, D.; Kim, C.-E.; Kang, K.S.; Seo, C.-S.; Choi, Y.-K.
- Issue Date
- Sep-2020
- Publisher
- MDPI AG
- Keywords
- Apoptosis; Breast cancer; Gallic acid; MCF-7; Network pharmacology; p53; Spatholobi Caulis
- Citation
- Processes, v.8, no.9
- Journal Title
- Processes
- Volume
- 8
- Number
- 9
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/78884
- DOI
- 10.3390/PR8091193
- ISSN
- 2227-9717
- Abstract
- Breast cancer is the most common malignancy in both developing and developed countries. In this study, we simultaneously analyzed nine constituent compounds from Spatholobi Caulis (gallic acid, (-)-gallocatechin, 3,4-dihydroxybenzoic acid, procyanidin B1, 3,4-dihydroxybenzaldehyde, catechin, procyanidin B2, epicatechin, and (-)-epicatechin gallate) and examined their anticancer effects on MCF-7 and MDA-MB-231 human breast cancer cells. The experimental results indicated that the gallic acid showed the strongest cytotoxic effect on MCF-7 cells among tested compounds whilst most of samples did not express inhibitory effect on viability of MDA-MB-231 cells, except for 70% ethanol extract of S. Caulis. Thus, gallic acid was chosen to extend anticancer mechanism study on MCF-7 cells. Our data showed that the gallic acid induced apoptotic MCF-7 cell death through both extrinsic and intrinsic pathways, which increased the expression of cleaved caspase-7, -8, and -9, Bax and p53, but reduced the expression of Bcl-2 and poly (ADP-ribose) polymerase (PARP). In addition, the network pharmacological analysis pointed out that the p53, mitogen-activated protein kinase (MAPK), estrogen, and Wnt signaling pathways have a great correlation with the targets of gallic acid. This study suggested that gallic acid is a bioactive component of S. Caulis with potential to be used in chemotherapy for breast cancer. © 2020 by the authors.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 한의과대학 > 한의학과 > 1. Journal Articles
- 한의과대학 > 한의예과 > 1. Journal Articles
- 바이오나노대학 > 생명과학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/78884)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.