Ventx1.1 competes with a transcriptional activator Xcad2 to regulate negatively its own expression
- Authors
- Kumar, Shiv; Umair, Zobia; Kumar, Vijay; Lee, Unjoo; Choi, Sun-Cheol; Kim, Jaebong
- Issue Date
- Jun-2019
- Publisher
- KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
- Keywords
- Autoregulation; Transcription; Ventx1.1; Xcad2; Xenopus
- Citation
- BMB REPORTS, v.52, no.6, pp.403 - 408
- Journal Title
- BMB REPORTS
- Volume
- 52
- Number
- 6
- Start Page
- 403
- End Page
- 408
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/81319
- DOI
- 10.5483/BMBRep.2019.52.6.085
- ISSN
- 1976-6696
- Abstract
- Dorsoventral patterning of body axis in vertebrate embryo is tightly controlled by a complex regulatory network of transcription factors. Ventx1.1 is known as a transcriptional repressor to inhibit dorsal mesoderm formation and neural differentiation in Xenopus. In an attempt to identify, using chromatin immunoprecipitation (ChIP)-Seq, genome-wide binding pattern of Ventx1.1 in Xenopus gastrulae, we observed that Ventx1.1 associates with its own 5'-flanking sequence. In this study, we present evidence that Ventx1.1 binds a cis-acting Ventx1.1 response element (VRE) in its own promoter, leading to repression of its own transcription. Site-directed mutagenesis of the VRE in the Ventx1.1 promoter significantly abrogated this inhibitory autoregulation of Ventx1.1 transcription. Notably, Ventx1.1 and Xcad2, an activator of Ventx1.1 transcription, competitively co-occupied the VRE in the Ventx1.1 promoter. In support of this, mutation of the VRE down-regulated basal and Xcad2-induced levels of Ventx1.1 promoter activity. In addition, overexpression of Ventx1.1 prevented Xcad2 from binding to the Ventx1.1 promoter, and vice versa. Taken together, these results suggest that Ventx1.1 negatively regulates its own transcription in competition with Xcad2, thereby fine-tuning its own expression levels during dorsoventral patterning of Xenopus early embryo.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 의과대학 > 의예과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/81319)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.