Anti-inflammatory effects of oroxylin A on RAW 264.7 mouse macrophages induced with polyinosinic-polycytidylic acid
- Authors
- Lee, Ji Young; Park, Wansu
- Issue Date
- Jul-2016
- Publisher
- SPANDIDOS PUBL LTD
- Keywords
- oroxylin A; double stranded-RNA; inflammation; macrophages; nitric oxide; cytokine; calcium; signal transducer and activated transcription 1
- Citation
- EXPERIMENTAL AND THERAPEUTIC MEDICINE, v.12, no.1, pp.151 - 156
- Journal Title
- EXPERIMENTAL AND THERAPEUTIC MEDICINE
- Volume
- 12
- Number
- 1
- Start Page
- 151
- End Page
- 156
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8138
- DOI
- 10.3892/etm.2016.3320
- ISSN
- 1792-0981
- Abstract
- Oroxylin A (5,7dihydroxy-6-methoxy-2-phe-nylchromen-4-one; Baicalein 6-methyl ether) is an active flavonoid compound originally isolated from Scutellaria radix, which has been used to treat pulmonary infection in Korea, China, and Japan. Oroxylin A is known to possess dopamine reuptake inhibitor activity. However, the effects of oroxylin A on virus-induced macrophages has not been fully elucidated. In the present study, the anti-inflammatory effects of oroxylin A on double-stranded RNA-induced macrophages were examined. Production of nitric oxide (NO), various cytokines, as well as calcium release and the mRNA expression of signal transducer and activator of transcription 1 (STAT1) in dsRNA polyinosinic-polycytidylic acid (PIC) -induced RAW 264.7 mouse macrophages were evaluated. Oroxylin A restored the cell viability in PIC-induced RAW 264.7 mouse macrophages at concentrations of up to 50 mu M. Additionally, oroxylin A significantly inhibited the production of nitric oxide (NO), interleukin (IL) -1 alpha, IL-1 beta, IL-6, IL-10, interferon gamma-induced protein 10, granulocyte-colony stimulating factor (CSF), granulocyte macrophage-CSF, leukemia inhibitory factor (IL-6 class cytokine), lipopolysaccharide-induced CXC chemokine (LIX), monocyte chemoattractant protein 1, macrophage inflammatory protein (MIP) -1 alpha, MIP-1 beta, MIP-2, Regulated on Activation, Normal T Expressed and Secreted, tumor necrosis factor-a, and vascular endothelial growth factor as well as calcium release and the mRNA expression of STAT1 in PIC-induced RAW 264.7 cells (P<0.05). Thus, the present results suggest that oroxylin A has anti-inflammatory properties, associated with its inhibition of NO, cytokines, chemokines and growth factors in PIC-induced macrophages via the calcium-STAT pathway.
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