Microfluidic electrical cell lysis for high-throughput and continuous production of cell-free varicella-zoster virus
- Authors
- Won, Eun-Jae; Thai, Duc Anh; Duong, Duong Duy; Lee, Nae Yoon; Song, Yoon-Jae
- Issue Date
- 20-Jul-2021
- Publisher
- ELSEVIER
- Keywords
- Cell-free VZV; Electrolysis; Focused electric field; Microfluidics
- Citation
- Journal of Biotechnology, v.335, pp.19 - 26
- Journal Title
- Journal of Biotechnology
- Volume
- 335
- Start Page
- 19
- End Page
- 26
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/81599
- DOI
- 10.1016/j.jbiotec.2021.06.006
- ISSN
- 0168-1656
- Abstract
- Varicella-zoster virus (VZV), the causative agent of varicella and herpes zoster, is highly cell-associated and spreads via cell-to-cell contact in tissue culture. The lack of cell-free VZV hampers studies on VZV biology as well as antiviral and vaccine development. In the present study, a poly(methylmethacrylate) microfluidic device integrated with arrays of microelectrode was fabricated to continuously electrolyse VZV-infected cells to produce cell-free viruses. By designing multiple constrictions and microelectrode arrays, a high electric field is focused on the constricted region of the microchannel to disrupt large numbers of virus-infected cells with high-throughput on a microfluidic platform. Plaque assay and scanning electron microscopy were conducted to quantify and characterize cell-free VZV produced using the microfluidic continuous-flow electrical cell lysis device. The process of microfluidic electrical cell lysis followed by subsequent filtration and virus concentration process yielded a 1.4–2.1 × 104 plaque-forming units (PFUs) per mL of cell-free VZV from 7.0 × 106 VZV-infected human foreskin fibroblasts (HFF) cells. The high electric field formed inside a microfluidic channel combined with the continuous-flow of virus-infected cells within the microchannel enabled the rapid and efficient production of high-titer cell-free virus in large quantities with relatively low input of the voltage. © 2021 Elsevier B.V.
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