Ultrasensitive biosensors based on waveguide-coupled long-range surface plasmon resonance (WC-LRSPR) for enhanced fluorescence spectroscopy
- Authors
- Thi, Tran N.H.; Phung, V.-D.; Thi, Ta H.K.; Lam, V.D.; Manh, D.H.; Pham, N.K.; Kim, Jae Young; Lee, Nae Yoon; Phan, B.T.
- Issue Date
- 3-Jul-2021
- Publisher
- Royal Society of Chemistry
- Citation
- RSC Advances, v.11, no.36, pp.22450 - 22460
- Journal Title
- RSC Advances
- Volume
- 11
- Number
- 36
- Start Page
- 22450
- End Page
- 22460
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/81676
- DOI
- 10.1039/d1ra02130c
- ISSN
- 2046-2069
- Abstract
- We investigated the coupling phenomenon between plasmonic resonance and waveguide modes through theoretical and experimental parametric analyses on the bimetallic waveguide-coupled long-range surface plasmon resonance (Bi-WCLRSPR) structure. The calculation results indicated that the multi-plasmonic coupling gives rise to the enhanced depth-to-width ratio of the reflection dip compared to that of LRSPR excited using a single set of Ag and Teflon. The optimized thickness of Ag(40 nm)/Teflon(700 nm)/Ag(5 nm)/Au(5 nm) was obtained and generated the highest plasmon intensity enhancement, which was 2.38 folds in comparison to the conventional bimetallic surface plasmon resonance (SPR) configuration (Ag/Au). 17β-Estradiol was used in the fluorescence enhancement experiment by the reflection geometry-based system, wherein the excitation light source was on the side of a WC-LRSPR chip opposite to that of the light detection unit. The phenomenon of surface plasmon-couple emission (SPCE) depends on the number of 17β-estradiol molecule promoters from female sex steroid hormones, which demonstrated a limit of detection (LOD) of 2 pg mL−1and 1.47-fold fluorescence improvement as compared to the non-coated material on the surface of pristine glass. This enhanced WC-LRSPR can readily find application in fluorescence escalation needed in cases where a weak fluorescence signal is predicted, such as the small volume of liquid containing fluorescent dyes in biological diagnosis. © The Royal Society of Chemistry 2021.
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