Development of a Multi-Layer Skin Substitute Using Human Hair Keratinic Extract-Based Hybrid 3D Printing
- Authors
- Choi, Won Seok; Kim, Joo Hyun; Ahn, Chi Bum; Lee, Ji Hyun; Kim, Yu Jin; Son, Kuk Hui; Lee, Jin Woo
- Issue Date
- Aug-2021
- Publisher
- MDPI
- Keywords
- 3D printing; Co-culture; Electrospinning; Human hair keratinic extract; Skin substitute
- Citation
- Polymers, v.13, no.16
- Journal Title
- Polymers
- Volume
- 13
- Number
- 16
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/82071
- DOI
- 10.3390/polym13162584
- ISSN
- 2073-4360
- Abstract
- Large-sized or deep skin wounds require skin substitutes for proper healing without scar formation. Therefore, multi-layered skin substitutes that mimic the genuine skin anatomy of multiple layers have attracted attention as suitable skin substitutes. In this study, a novel skin substitute was developed by combining the multi-layer skin tissue reconstruction method with the combination of a human-derived keratinic extract-loaded nano-and micro-fiber using electrospinning and a support structure using 3D printing. A polycaprolactone PCL/keratin electrospun scaffold showed better cell adhesion and proliferation than the keratin-free PCL scaffold, and keratinocytes and fibroblasts showed better survival, adhesion, and proliferation in the PCL/keratin electrospun nanofiber scaffold and microfiber scaffold, respectively. In a co-culture of keratinocytes and fibroblasts using a multi-layered scaffold, the two cells formed the epidermis and dermal layer on the PCL/keratin scaffold without territorial invasion. In the animal study, the PCL/keratin scaffold caused a faster regeneration of new skin without scar formation compared to the PCL scaffold. Our study showed that PCL/keratin scaffolds co-cultured with keratinocytes and fibroblasts promoted the regeneration of the epidermal and dermal layers in deep skin defects. Such finding suggests a new possibility for artificial skin production using multiple cells. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 의과대학 > 의예과 > 1. Journal Articles
- 의과대학 > 의학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/82071)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.