우고닌(Wogonin)이 poly I:C로 유발된 TM4세포 내하이드로겐퍼록사이드 생성에 미치는 영향Effect of Wogonin on Intracellular Hydrogen Peroxide Productionof TM4 Mouse Sertoli cells stressed with polyinosinic:polycytidylic acid
- Other Titles
- Effect of Wogonin on Intracellular Hydrogen Peroxide Productionof TM4 Mouse Sertoli cells stressed with polyinosinic:polycytidylic acid
- Authors
- 박완수
- Issue Date
- Sep-2021
- Publisher
- 대한본초학회
- Keywords
- Sertoli Cell; TM4; poly I:C; Wogonin; Hydrogen Peroxide; Reactive Oxygen Species
- Citation
- 대한본초학회지, v.36, no.5, pp.117 - 123
- Journal Title
- 대한본초학회지
- Volume
- 36
- Number
- 5
- Start Page
- 117
- End Page
- 123
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/82263
- ISSN
- 1229-1765
- Abstract
- Objectives : The aim of this study is to investigate the effect of wogonin on the production of hydrogen peroxide in polyinosinic:polycytidylic acid (poly I:C)-stimulated TM4 mouse sertoli cells.
Methods : TM4 were treated with poly I:C (50 ug/mL) and wogonin at concentrations of 5, 10, 25, and 50 µM for 30 min, 2 hr, 12 hr, 18 hr, and 24 hr. The production of intracellular hydrogen peroxide was measured by dihydrorhodamine 123 assay.
Results : For 30 min, 2 hr, 12 hr, 18 hr, and 24 hr treatment, wogonin significantly inhibited intracellular hydrogen peroxide productions of TM4 at the concentration of 5, 10, 25, and 50 µM (p <0.05). In details, production of hydrogen peroxide in poly I:C-stimulated TM4 treated for 30 min with wogonin at concentrations of 5, 10, 25, and 50 µM was 95.67%, 92.69%, 92.05%, and 91.97% of the control group treated with poly I:C only, respectively; the production of hydrogen peroxide for 2 hr was 94.44%, 94.41%, 93%, and 92.98%, respectively; production of hydrogen peroxide for 12 hr was 96.78%, 95.32%, 94.33%, and 93.17%, respectively; production of hydrogen peroxide for 18 hr was 94.7%, 93.4%, 93.38%, and 93.35%, respectively; and production of hydrogen peroxide for 24 hr was 95.75%, 94.77%, 94.58%, and 92.8%, respectively.
Conclusions : Wogonin might have anti-viral property related with its inhibition of intracellular hydrogen peroxide production in poly I:C-stimulated TM4 cells.
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