A simple and innovative sample preparation method for on-site SARS-CoV-2 molecular diagnostics
- Authors
- Lee, Songhyun; Song, Junkyu; Kim, Sanghyo
- Issue Date
- Nov-2021
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- ANALYST, v.146, no.22, pp.6917 - 6923
- Journal Title
- ANALYST
- Volume
- 146
- Number
- 22
- Start Page
- 6917
- End Page
- 6923
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/82650
- DOI
- 10.1039/d1an01401c
- ISSN
- 0003-2654
- Abstract
- Nucleic acid amplification is a widely used diagnostic tool, although it requires a relatively time-consuming and complicated extraction step. To address this issue outside the laboratory, we investigated a sample preparation system and determined that a silica membrane and silica-coated beads are powerful tools for the extraction from raw samples: nucleic acids are kept in the silica membrane, retained during a single wash step, and released at the elution step. The eluent is appropriate for the quantitative real-time polymerase chain reaction (qPCR) and loop-mediated amplification (LAMP) assay in terms of purity and quantity. We also built an innovative equipment-free nucleic acid extraction squeeze system which requires less than 20 min. The sample with improved purity augments the specificity and sensitivity. This system is simple, user-friendly, low-cost, and equipment-free, thus making nucleic acid extraction more accessible and affordable for researchers and untrained users. Furthermore, when combined with the reverse-transcription quantitative real-time polymerase chain reaction method, the method will accelerate the detection of diseases. The same goes when combined with the LAMP assay, especially in developing countries.
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