Schisandrin C Affects Glucose-Stimulated Insulin Secretion in Pancreatic beta-Cells and Glucose Uptake in Skeletal Muscle Cells
- Authors
- Lee, Dahae; Kim, Young-Mi; Kim, Hyun Woo; Choi, You-Kyoung; Park, Bang Ju; Joo, Sang Hoon; Kang, Ki Sung
- Issue Date
- Nov-2021
- Publisher
- MDPI
- Keywords
- schisandrin C; glucose-stimulated insulin secretion; glucose uptake; PDX-1; GLUT-4
- Citation
- MOLECULES, v.26, no.21
- Journal Title
- MOLECULES
- Volume
- 26
- Number
- 21
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/82779
- DOI
- 10.3390/molecules26216509
- ISSN
- 1420-3049
- Abstract
- The aim of our study was to investigate the effect of three lignans (schisandrol A, schisandrol B, and schisandrin C) on insulin secretion in rat INS-1 pancreatic beta-cells and glucose uptake in mouse C2C12 skeletal muscle cells. Schisandrol A and schisandrin C enhanced insulin secretion in response to high glucose levels with no toxic effects on INS-1 cells. The effect of schisandrin C was superior to that of gliclazide (positive control), a drug commonly used to treat type 2 diabetes (T2D). In addition, western blot analysis showed that the expression of associated proteins, including peroxisome proliferator-activated receptor gamma (PPAR gamma), pancreatic and duodenal homeobox 1 (PDX-1), phosphatidylinositol 3-kinase (PI3K), Akt, and insulin receptor substrate-2 (IRS-2), was increased in INS-1 cells after treatment with schisandrin C. In addition, insulin secretion effect of schisandrin C were enhanced by the Bay K 8644 (L-type Ca2+ channel agonist) and glibenclamide (K+ channel blocker), were abolished by the nifedipine (L-type Ca2+ channel blocker) and diazoxide (K+ channel activator). Moreover, schisandrin C enhanced glucose uptake with no toxic effects on C2C12 cells. Western blot analysis showed that the expression of associated proteins, including insulin receptor substrate-1 (IRS-1), AMP-activated protein kinase (AMPK), PI3K, Akt, glucose transporter type 4 (GLUT-4), was increased in C2C12 cells after treatment with schisandrin C. Schisandrin C may improve hyperglycemia by enhancing insulin secretion in pancreatic beta-cells and improving glucose uptake into skeletal muscle cells. Our findings may provide evidence that schisandrin C may be beneficial in devising novel anti-T2D strategies.
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