Thymol and eugenol in essential oils enhance phage endolysin LysECP26-mediated cell wall disruption of Escherichia coli O157:H7
- Authors
- Park, Do-Won; Lee, Jong Hun; Park, Jong-Hyun
- Issue Date
- Dec-2021
- Publisher
- 한국식품과학회
- Keywords
- bacteriophage; endolysin; thymol; eugenol; Esherichia coli O157:H7
- Citation
- 한국식품과학회지, v.53, no.6, pp.819 - 822
- Journal Title
- 한국식품과학회지
- Volume
- 53
- Number
- 6
- Start Page
- 819
- End Page
- 822
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/84195
- DOI
- 10.9721/KJFST.2021.53.6.819
- ISSN
- 0367-6293
- Abstract
- To enhance phage endolysin-mediated cell wall disruption of Escherichia coli O157:H7, the cells were cotreated with aromatic compounds, namely thymol or eugenol, found in essential oils and endolysin LysECP26.
Interestingly, the minimal inhibitory concentrations of LysECP26 was four times lower when used in combination with either of the two compounds than when it was used alone. This synergistic activity was also confirmed by viable cell counting. Within 1 h of LysECP26 and eugenol or thymol co-treatment to the cells, there was a 2.3 or 3.8 log CFU/mL reductions, respectively. Additionally, field emission scanning electron microscopy showed cell wall disruption and severe morphological alterations of the cells in case of the combination treatments. Therefore, endolysin and thymol or eugenol co-treatment can help in developing efficient bio-control strategies against gram-negative pathogen E. coli O157:H7.
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Collections - 바이오나노대학 > 식품생물공학과 > 1. Journal Articles
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