Effects of Bacillus Subtilis-Fermented White Sword Bean Extract on Adipogenesis and Lipolysis of 3T3-L1 Adipocytesopen access
- Authors
- Choi, Yujeong; Kim, Da-Som; Lee, Min-Chul; Park, Seulgi; Lee, Joo-Won; Om, Ae-Son
- Issue Date
- Jun-2021
- Publisher
- MDPI
- Keywords
- Canavalia gladiata; triglyceride; glycerol; AMP-activated protein kinase; peroxisome proliferator-activated receptor
- Citation
- FOODS, v.10, no.6
- Journal Title
- FOODS
- Volume
- 10
- Number
- 6
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/84684
- DOI
- 10.3390/foods10061423
- ISSN
- 2304-8158
- Abstract
- To investigate the adipogenesis and lipolysis effects of the Bacillus subtilis-fermented white sword bean extract (FWSBE) on 3T3-L1 adipocytes, we treated 3T3-L1 preadipocytes before and after differentiation with FWSBE and measured triglyceride, free glycerol, mRNA, and protein levels. First, FWSBE reduced the cell viability of 3T3-L1 pre-adipocytes under 1000 mu g/mL conditions. Triglyceride accumulation in 3T3-L1 pre-adipocytes was suppressed, and free glycerol content in mature 3T3-L1 adipocytes was increased in the FWSBE treatment groups, indicating that FWSBE has anti-obesity effects. Further, FWSBE suppressed adipogenesis in 3T3-L1 pre-adipocytes by lowering the protein levels of C/EBP alpha, PPAR gamma, and FAS and increasing the level of pACC and pAMPK. Additionally, FWSBE promoted lipolysis in mature 3T3-L1 adipocytes by increasing the transcription levels of Ppara, Acox, and Lcad and the protein levels of pHSL and ATGL. Thus, we suggest that FWSBE can be a potential dietary supplement because of its anti-obesity properties.
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