Comparison of the antiviral activity of flavonoids against murine norovirus and feline calicivirus

Abstract

Antiviral effect of 10 flavonoids against feline calicivirus (FCV) and murine norovirus (MNV) was investigated on Crandell-Reese feline kidney (CRFK) cells and RAW 264.7 cells. Each cells infected with FCV or MNV was treated with different flavonoids at concentrations between 50 and 300 mu M. A 200 mu M dose of kaempferol and daidzein significantly reduced the FCV titer by 69.76 +/- 8.71 and 63.47 +/- 9.82%, respectively. A 300 mu M dose of quercetin and fisetin reduced the titer of FCV titer by 56.70 +/- 11.39 and 41.84 +/- 11.35%, respectively. The titer of MNV was reduced by 50.47 +/- 6.91 and 51.21 +/- 13.38% by a 50 mu M dose of fisetin or a 150 mu M dose of epicatechin gallate, respectively. A 50 mu M dose of daidzein and 100 mu M of quercetin reduced the MNV titer by 46.32 +/- 8.70 and 45.78 +/- 9.81%, respectively. The pre-treatment of quercetin, fisetin, daidzein, and epicatechin gallate demonstrated the anti-noroviral activity against MNV and FCV. (C) 2015 Elsevier Ltd. All rights reserved.