Effects of erythropoietin on osteoblast in the tooth extraction socket in mice periodontitis modelopen access
- Authors
- Bae, Ju-Eun; Hwang, Sung-Min; Aryal, Yam Prasad; Kim, Tae-Young; Sohn, Wern-Joo; An, Seo-Young; Kim, Ji-Youn; An, Chang-Hyeon; Lee, Youngkyun; Kim, Yong-Gun; Park, Jin-Woo; Lee, Jae-Mok; Kim, Jae-Young; Suh, Jo-Young
- Issue Date
- Oct-2022
- Publisher
- FRONTIERS MEDIA SA
- Keywords
- human periodontal ligament fibroblast cell; local delivery; MC3T3-E1 cells; osteoblast; periodontitis; tooth loss
- Citation
- FRONTIERS IN PHYSIOLOGY, v.13
- Journal Title
- FRONTIERS IN PHYSIOLOGY
- Volume
- 13
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/86036
- DOI
- 10.3389/fphys.2022.987625
- ISSN
- 1664-042X
- Abstract
- Periodontitis is an excessive inflammatory event in tooth-supporting tissues and can cause tooth loss. We used erythropoietin (EPO), which has been reported to play an important role in bone healing and modulation of angiogenesis, as a therapeutic agent in vivo and in vitro experimental models to analyze its effect on periodontitis. First, EPO was applied to in vitro MC3T3-E1 cells and human periodontal ligament fibroblast (hPDLF) cells to examine its function in altered cellular events and gene expression patterns. In vitro cultivation of MC3T3-E1 and hPDLF cells with 10 IU/ml EPO at 24 and 48 h showed an obvious increase in cell proliferation. Interestingly, EPO treatment altered the expression of osteogenesis-related molecules, including alkaline phosphatase (ALP), bone morphogenetic protein-2 (BMP-2), and osteocalcin (OC) in MC3T3-E1 cells but not in hPDLF cells. In particular, MC3T3-E1 cells showed increased expression of ALP, BMP-2, and OC on day 5, while hPDLF cells showed increased expression of BMP-2, and OC on day 14. Based on the in vitro examination, we evaluated the effect of EPO on bone formation using an experimentally-induced animal periodontitis model. After the induction of periodontitis in the maxillary left second M, 10 IU/ml of EPO was locally applied to the extraction tooth sockets. Histomorphological examination using Masson's trichrome (MTC) staining showed facilitated bone formation in the EPO-treated groups after 14 days. Similarly, stronger positive reactions against vascular endothelial growth factor (VEGF), cluster of differentiation 31 (CD31), runt-related transcription factor 2 (RUNX2), and osteocalcin (OC) were detected in the EPO-treated group compared to the control. Meanwhile, myeloperoxidase, an inflammatory marker, was decreased in the EPO-treated group on days 1 and 5. Overall, EPO facilitates bone healing and regeneration through altered signaling regulation and modulation of inflammation in the osteoblast cell lineage and to a lesser extent in hPDLF cells.
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