Bifidogenic property of enzymatically synthesized water-insoluble alpha-glucans with different alpha-1,6 branching ratio
- Authors
- Ryu, Hye-Jung; Jung, Dong-Hyun; Yoo, Sang-Ho; Tuncil, Yunus E.; Lee, Byung-Hoo
- Issue Date
- Dec-2022
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Amylosucrase; Glycogen branching enzyme; Insoluble alpha-glucans; Resistant starch; Bifidogenic property
- Citation
- FOOD HYDROCOLLOIDS, v.133
- Journal Title
- FOOD HYDROCOLLOIDS
- Volume
- 133
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/86636
- DOI
- 10.1016/j.foodhyd.2022.107987
- ISSN
- 0268-005X
- Abstract
- Bifidobacteria uses insoluble resistant starch (RS) fractions as prebiotics in the colon, consequently improving human intestinal health. In this study, various alpha-1,6 linkage ratios of insoluble alpha-glucans were synthesized by amylosucrase from Neisseria polysaccharea and glycogen branching enzyme from Rhodothermus obamensis to investigate the effect of branching patterns on Bifidogenic property. Different ratios (0%-4%) of alpha-1,6 branched insoluble alpha-glucans with alpha-1,4 linked backbones were effectively synthesized. Additionally, the proportions of RS fraction in the synthesized alpha-glucans were 69.5%-81.9% after the cooking process, which are higher amounts than amylomaize VII (36.8%). As various Bifidobacteria used the insoluble branched alpha-glucans, the structure with 2.5% of 1,6 linkage showed the highest consumption yield, more than 70%, among all tested strains. Therefore, the insoluble alpha-glucans with different alpha-1,6 linkage ratios can be applied as prebiotic ingredients to modulate the Bifidobacterium species to enhance colon health and related outcomes.
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Collections - 바이오나노대학 > 식품생물공학과 > 1. Journal Articles
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