A novel paper-based lysis strip for SARS-CoV-2 RNA detection at low resource settingsopen access
- Authors
- Lee, Songhyun; Kim, SeJin; Kim, Sanghyo
- Issue Date
- Mar-2023
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- ARS-CoV-2; RNA extraction; Lysis; Point of care test; Paper-based
- Citation
- ANALYTICAL BIOCHEMISTRY, v.664
- Journal Title
- ANALYTICAL BIOCHEMISTRY
- Volume
- 664
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/87066
- DOI
- 10.1016/j.ab.2023.115037
- ISSN
- 0003-2697
- Abstract
- Infectious respiratory diseases such as COVID-19 are serious and global concerns from the past to the present. To isolate the spread of infectious diseases even in the absence of a health system, a simple, inexpensive, reliable, sensitive, and selective molecular diagnosis platform for Point of Care Test (POCT) is required. Especially, the nucleic acid extraction step is difficult to perform out of laboratory. Here, we propose a paper-based lysis (PBL) strip for nucleic acid extraction, especially in low-resource settings (LRS). PBL strips are suitable for isolating RNA from viruses with biological interference and inhibitors. We optimized the buffer compositions and membranes of the strip. A simple preparation method using a PBL strip could obtain an eluent for downstream inspection within 20 min. Overall, 104 copies/swaps were detected for 20 min for amplification in combination with Reverse Transcription Loop-Mediated Amplification (RT-LAMP).
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