Hierarchical Au nanoclusters electrodeposited on amine-terminated ITO glass as a SERS-active substrate for the reliable and sensitive detection of serotonin in a Tris-HCl buffer solution
- Authors
- Viet-Duc Phung; Kook, Jeong-Keun; Koh, Do Yeung; Lee, Sang-Wha
- Issue Date
- 14-Nov-2019
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- DALTON TRANSACTIONS, v.48, no.42, pp.16026 - 16033
- Journal Title
- DALTON TRANSACTIONS
- Volume
- 48
- Number
- 42
- Start Page
- 16026
- End Page
- 16033
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/872
- DOI
- 10.1039/c9dt03269j
- ISSN
- 1477-9226
- Abstract
- Although serotonin (5-HT) is one of the most important neurotransmitters that are responsible for critical roles in the central nervous system, only few studies have been reported on the reliable and sensitive detection of 5-HT by the SERS technique owing to the lower Raman cross-section and lack of a common extraction method of 5-HT. In this study, a SERS-active substrate was fabricated by electrodepositing hierarchical Au nanostructures on amine-terminated ITO (indium tin oxide) glass to achieve an enhanced Raman signal of 5-HT. The electrodeposition process was performed by applying the nucleation potential E-1 = 0.7 V for 2 s and the growth potential E-2 = -0.2 V for 1000 s for repeated cycles. As a testing analyte, 5-HT was dissolved in four different solvents i.e. PBS buffer, Tris-HCl buffer, distilled water, and ethanol solution (20%). Among them, Tris-HCl buffer produced the most sensitive and reproducible Raman signals for 5-HT along with the lowest detection limit of similar to 10(-9) M due to the strong adsorption interactions between the Au surface and the indole amine groups of 5-HT under alkaline conditions. Surprisingly, the standard curve between the Raman intensity at 751 cm(-1) and the log-scale concentration of 5-HT showed a linear dynamic range of over five orders of magnitude in the Tris-HCl buffer solution, suggesting a possible quantification of the 5-HT level in a biological fluid with 5-HT dysfunction or deficiency.
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